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Topflash实验方法

WebDay 1 : See 0.2e6 HEK293 Top Flash cells (stable cell lines with Renilla/Luc reporters) in 250uL DMEM-10% FBS, on a 24-well plate. Day 2 : Change media with 0, 50, 100, 200, 300, 400 and 500ng/ml... Web16. máj 2024 · Technology Overview Researchers at Johns Hopkins have developed a HEK293 cell line that is a stable reporter of canonical Wnt signaling. The cell line, which contains a luciferase reporter under control of 7 LEF/TCF binding sites, utilizes the Super TOPFlash system.

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Web21. feb 2012 · TOPflash and FOPflash constructs are widely used to evaluate β-catenin-dependent signaling events that drive the expression of TCF. TOPflash is a TCF reporter … Web25. máj 2024 · 载体描述. Transfection grade T cell factor (Tcf) reporter plasmid containing three copies of the Tcf binding site (mutated) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPFLASH (Catalog # 21-170) which contains wild type Tcf binding sites. how much storage does ddlc take up https://aweb2see.com

molecular biology - How does a TOPflash/FOPflash assay work to …

Web21. feb 2012 · 具体的TOP或FOP质粒与海肾荧光素载体的比例，需要个人通过实验优化，通常取值的范围在10/1到50/1。 数据处理： 通常以TOP/FOP的比值表示。 公式如下： 转染有TOP-Flash和海肾荧光素载体的样本中采集得到的萤火虫荧光值F (Top)/采集得到的海肾荧光值R (Top) 本回答被网友采纳 92 评论 分享 举报 shovel39 2012-02-21 · TA获得超过345个 … Web具体的TOP或FOP质粒与海肾荧光素载体的比例，需要个人通过实验优化，通常取值的范围在10/1到50/1。 数据处理： 通常以TOP/FOP的比值表示。 公式如下： 转染有TOP-Flash和 … WebTOPflash: Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild … men\u0027s all white designer sneakers

TOP/FOP-FLASH assay in HEK293T cells? ResearchGate

Category:FOPFlash说明书-分析方法-生物在线 Lab-on-Web - BIOON

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Topflash实验方法

wnt通路中，TOPflash/FOPflash有啥区别？-盖德化工问答

WebResearchers who want to detect active Wnt-ßcatenin signaling in cells have various tools available. To measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from various sources including lentivirus-based reporters from the Nusse and ... Web1.应该是要用Top/FOP值来表示结果，Fop值当作一个背景值。 2.Fop是比较稳定的，特别是在组内。 不过你说“各组之间”，有可能不同处理会有影响，不过应该不影响结果分析。 一 …

Topflash实验方法

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WebM50 Super 8x TOPFlash Sequences (3) Addgene Sequences: Full (1) Partial (2) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). ... WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF …

WebIn our lab, we usually transfect 50ng of DNA per well of a 96 well plate (in which we plate between 10-30K cells, depending cell line). From that 50ng DNA, 15ng are TOPFlash … Web1. sep 2015 · 1.应该是要用Top/FOP值来表示结果，Fop值当作一个背景值。 2.Fop是比较稳定的，特别是在组内。 不过你说“各组之间”，有可能不同处理会有影响，不过应该不影响结果分析。 一般Top会比Fop高或相似，除非你的处理是抑制Wnt通路的。 3.如果Fop值有降低，应该不是Wnt通路的改变引起的。 Top和Fop除了Wnt的反应元件不同，其余都一样， …

Web25. máj 2024 · Transfection grade T cell factor (Tcf) reporter plasmid containing three copies of the Tcf binding site (mutated) upstream of the Thymidine Kinase (TK) minimal … WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame. - Find MSDS or SDS, a COA, data sheets and more information.

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http://www.bioon.com.cn/doc/showarticle.asp?newsid=96515 how much storage does fifa 22 useWeb用western和免疫荧光验证beta－catenin和下游靶基因的变化只是基础，只是说明你转染了这个抑癌基因的确是改变了catenin通路，但是你想要证明该抑癌基因是通过catenin通路来 … how much storage does discord takeWebTOPflash: Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild type) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. FOPflash containing mutated TCF binding sites is also available as a ... how much storage does brawlhalla take