Raw-blue cells
WebApr 22, 2015 · S2 Fig. RAW-BLUE cells detect trace levels of endotoxin in heparin lots. RAW-BLUE cells were cultured with 10 different lots of heparin (#1–10) in the presence or absence of 100pg/mL endotoxin for 24h. TNF-α levels were measured by ELISA in cell supernatant at 24 h. Each point represents mean ± SD of triplicate cell culture. WebFeb 17, 2024 · Coculture D1 Cells:RAW-Blue Cells Additionally, we prepared cocultures of D1 and RAW-blue cells using transwell inserts without the Dex P-A hydrogel. After 24 h incubation at 37 °C, NF-κB/AP-1 activation upon the previously mentioned stimuli was assessed by measuring the release of secreted embryonic alkaline phosphates (SEAPs) …
Raw-blue cells
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WebNF-κB activity from LPS-activated RAW-Blue™ cells after incubation with 30 and 300 μg/ml of acacia (AG), 30 and 300 μg/ml of guar gum (GG) and 30 μg/ml of 5ASA for 24 h in serum-free medium ... WebRAW-Blue™ cells. Catalog No. raw-sp. NF-κB-SEAP Reporter Cell Line. Pack Size. 3-7 x 10e6 cells. Quantity.
WebAug 24, 2010 · RAW-Blue cells are derived from RAW-264.7 macrophages that stably express a secreted embroyonic alkaline phosphotase (SEAP) gene which can be induced by NF-κB or AP-1 transcription factors. Treatment of RAW-Blue cells with Rx inhibited the LPS-induced NF-κB activity in a time- and dose-dependent manner as indicated by the reduced … WebSep 30, 2024 · Supernatant from the treated cells was collected and SEAP production was detected over a period of 45 minutes at 655 nm using the QUANTI-Blue ™ detection medium. Detection of protein expression by immunoblotting and densitometry. Cells from the treated well-plate were lysed and saved in the -80 ° C freezer for
WebTherefore, RAW-Blue™ cells can be used as: TLR reporter cells: they express all TLRs, with the exception of TLR5, at different levels NOD reporter cells: … WebSep 5, 2024 · RAW-Blue cells and HEK 293 h-TLR transfected cells Murine RAW-Blue, HEK-BLUE-hTLR2 and HEK-BLUE-hTLR4 cells carrying a SEAP reporter construct were purchased from InvivoGen (San Diego, CA, USA).
WebSize. 3-7 x 10e6 cells. Contact us. Ask RAW ™ cells selection audit. Properties. Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water. Conjugation. Blue Substrate.
WebCo-immunoprecipitation experiments using cell lysates from RAW-blue cells further dem- onstrated that Neu1 forms a complex with TLR9 receptors in naive and ODN treatment of cells for 30 min (Fig. 4C). ciliary muscles are a type of skeletal muscleWebRAW 264.7. TIB-71 ™. RAW 264.7 is a macrophage cell line that was established from a tumor in a male mouse induced with the Abelson murine leukemia virus. Product category. dhl packstation trierWebRAW-blue cells were pretreated with 200μM Tamiflu, 100μg/mL MMP9i, or 100μg/mL BIM-23127 for 30min followed by 10μg/mL imiquimod or left cultured in media. Cells (1×107 cells) are pelleted ... dhl packstation ukWebRAW-Blue™ cells endongenously express various PRRs, including TLRs (except TLR5), RIG-I, MDA-5, NOD1/2 and Dectin-1 at different levels. The PRR stimulation using specific agonists leads to the activation of NF-kB and AP-1 and, subsequently to the expression of the reporter gene encoding SEAP. RAW-Blue™ cells are selectable with Zeocin®. ciliary muscle 中文WebThe in vitro model using RAW 264.7 cells has been widely used. However, there is still no consensus on which inflammatory mediators should initially be measured to screen for possible anti-inflammatory effects. To determine the rationality of measuring inflammatory mediators together with NO, such as the levels of tumor necrosis factor ... ciliary muscles in the eyeWebFeb 13, 2016 · Anti-inflammatory bioassay. RAW-Blue cells (5 × 10 4 /well) were cultured in 96-well plates for 24 h, and then treated with LPS (1 μg/mL) alone or together with test samples for 20 h. Secreted embryonic alkaline phosphatase (SEAP) activity in the medium was determined by QUANTI-Blue medium following the manufacturer’s instructions. ciliary plasm翻译WebNational Center for Biotechnology Information dhl packstation storkower str