Phosphinothricin-resistant

Author: ccec

August undefined, 2024

Glufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of Streptomyces soil bacteria. Glufosinate is a non-selective, contact herbicide, with some systemic action. Plants may also metabolize bialaphos and phosalacine, other naturally occurring herbicides, directly into glufosinate. The … WebCAS No. 7803-51-2. Phosphine (PH₃) is a colorless, flammable, and explosive gas at room …

Phosphinothricin-Resistant Crops - ResearchGate

WebJan 27, 2024 · The fast spread of bacteria that are resistant to many classes of antibiotics (multidrug resistant) is a global threat to human and animal health with a worrisome scenario ahead. Novel therapeutical strategies are … WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. how to set up apple music family sharing

A facile method for screening for phosphinothricin (PPT)-resistant ...

WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ... Webresistance of expiants to, phosphinothricin is presented. Key words: Ammonium - Carbon source - Glufosinate - Glutamine - Metabolic activity - Phosphinothricin Introduction Selectable marker genes are still a necessity in plant trans formation technology. In the presence of a selective agent, only those cells that are transformed and express the WebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) carfentrazone-ethyl in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions … nothilfe instruktor jobs

Federal Register, Volume 88 Issue 69 (Tuesday, April 11, 2024)

Efficient expression of heterologous genes by the introduction of …

WebNov 6, 2006 · Selection for kanamycin-, phosphinothricin- and hygromycin B-resistance commonly takes 7-10 d and high seedling density and fungal contamination may result in failure to recover transformants. Results: A method for identifying transformed seedlings in as little as 3.25 d has been developed. WebOct 23, 2015 · The R295K mutation has also never been previously reported as an … nothilfe im auslandWebA gene which confers resistance to the herbicide bialaphos (bar) has been characterized. The bar gene was originally cloned from Streptomyces hygroscopicus, an organism which produces the tripeptide bialaphos as a secondary metabolite. Bialaphos contains phosphinothricin, an analogue of glutamate which is an inhibitor of glutamine synthetase. nothilfe großhadern

"WebOct 23, 2015 · Increasing PPT-resistant gene diversity and generating multi-herbicide … " - Phosphinothricin-resistant

Phosphinothricin-resistant

Non-specific activities of the major herbicide-resistance gene BAR

WebA bioassay was developed for identifying transgenic wheat plants based on De Block et al.'s [8] ammonium-multiwell assay which allows qualitative and quantitative evaluation of the expression of the enzyme phosphinothricin acetyl transferase (PAT). Important parameters in the assay are the use of young leaf tissues, short incubation period (6 h) and a high … WebA bioassay was developed for identifying transgenic wheat plants based on De Block et …

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WebUnder optimised electroporation conditions, up to 0.9% of viable protoplasts displayed gus activity two days after electroporation. To select for phosphinothricin (PPT) resistant colonies, electroporated protoplasts were incubated for six weeks in a medium containing 10 μg/ml PPT. The cells surviving the selection were maintained as individual ... WebThese results indicated that the extra intron at the 5 0 end of the phosphinothricin-resistance gene is required for its efficient expression in G. lucidum. To confirm these results, we also ...

WebOct 1, 1996 · The enzymes have a similar substrate affinity towards L-phosphinothricin and do not acetylate any of the other L-amino acids tested. ... Engineering herbicide resistance in plants by expression of ... WebOct 4, 2009 · The transgenic plants were resistant to phosphinothricin up to 200 mg/l, …

WebJan 1, 2008 · Phosphinothricin resistance as selectable marker for genetic transformation … WebBialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research and genetically engineered crops1–4.

WebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl- (methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am Main, Germany), which is a nonselective, postemergence herbicide used for weed control in orchards and vineyards 1.

WebNov 27, 2024 · In the 1980s, the bialaphos resistance ( BAR) gene and its closely related … how to set up apple pay on iphone 10WebPhosphinothricin. Resistance against PTT can be achieved by elevated levels of GS, which … how to set up apple pay ipadWebJan 18, 2024 · One of the microbes that produce phosphinothricin, Streptomyces hygroscopicus, has an enzyme that inactivates phosphinothricin and glufosinate by acylating it. The gene (bar) encoding this... nothilfe im kinderspitalWebSep 12, 2015 · In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus reporter and a bar … how to set up apple pen with procreate pocketWebJul 14, 2024 · In the current study, a chloroplast transformation vector harboring the phosphinothricin resistance gene (bar) as a selectable marker was established, and two short peptide-encoding genes (ant1 and ant2) driven by the endogenous psbA and rbcL promoters were cloned in this vector. The recombinant plasmid was transformed using a … how to set up apple pay on iphone 7WebNov 6, 2006 · The phosphinothricin-resistant seedlings were uniformly much greener than … how to set up apple pay iphoneWebPhosphinothricin C5H11NO4P- CID 25244585 - structure, chemical names, physical and … nothilfe kiel