Host-pathogen transcriptomics by dual rna-seq

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August undefined, 2024

WebJun 5, 2024 · Dual RNA-seq applies deep sequencing to simultaneously quantify genome-wide transcriptional responses of host and pathogen 8,9. This approach offers higher efficiency and more restricted technical ... WebRNA-sequencing (RNA-seq) delivers genome-wide quantitative snapshots of the transcriptome [24]. In a thought experiment, Vogel and co-workers argued that simultaneous profiling of host and pathogen transcriptomics by dual RNA-seq might provide valuable insights for infection biology [7]. Recent dual RNA-seq studies were successful in elucidat-

Resolving host–pathogen interactions by dual RNA-seq

WebThe current unfeasibility of axenic culture and the low complexity of genomic sequences are obstacles in assembling complete chromosomes. Here, a method combining pathogen DNA enrichment from infected insects and dual deep-sequencing technologies was used to obtain the complete genome of a phytoplasma causing Grapevine Flavescence dorée. WebMay 9, 2013 · I am working on integrating multiple -omics technologies such as whole genome sequencing, bulk RNA sequencing, ChIP sequencing, single cell omics, proteomics, metabolomics, and microbiomics using ... dashmat covers amazon

Resolving host-pathogen interactions by dual RNA-seq

WebAn extensive body of evidence points toward the use of dual RNA-seq to elucidate molecular mechanisms exploited by diverse pathogens for remodeling of the host transcriptome (Westermann, Barquist and Vogel 2024 ). WebMar 26, 2024 · Experienced investigator with a demonstrated history of studying the host response to virus infection, with a focus on highly … WebA generic ‘dual RNA-seq’ approach is used to profile RNA expression simultaneously in pathogen and host during Salmonella enterica serovar Typhimurium infection and reveal … dash mandoline safe slice stores

Frontiers Dual Transcriptomic Analyses Unveil Host–Pathogen ...

Dual RNA-seq for host–pathogen transcriptomics - Nature

WebAug 8, 2024 · Numerous sequencing studies, including transcriptomics of host-pathogen systems, sequencing of hybrid genomes, xenografts, mixed species sys. Skip to Main Content. Advertisement. Journals. ... Resolving host–pathogen interactions by dual RNA-seq. PLoS Pathog., 13, e1006033. Google Scholar. WebFeb 16, 2024 · Abstract The transcriptome is a powerful proxy for the physiological state of a cell, healthy or diseased. As a result, transcriptome analysis has become a key tool in … maroccheWebAbstract RNA-sequencing technology has been widely adopted to investigate host responses during infection with pathogens. Dual RNA-sequencing (RNA-seq) allows the simultaneous capture of pathogen specific transcripts during infection, providing a more complete view of the interaction. dash medical supplies

"WebApr 30, 2015 · Further advances allow for simultaneous host-pathogen transcriptomics (dual-RNA-seq) (Westermann et al., 2012). As RNA-seq is based upon NGS, it can be applied in any biological context from which ... " - Host-pathogen transcriptomics by dual rna-seq

Host-pathogen transcriptomics by dual rna-seq

Dual RNA-Seq of Chlamydia and Host Cells - PubMed

WebAug 14, 2012 · Host or pathogen transcriptomics The first transcriptomic studies became possible with the development of cDNA microarrays 4, 5. This entailed the use of … WebDual RNA-Seq has been applied to simultaneously profile RNA expression in both the pathogen and host throughout the infection process. This technique enables the study of …

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WebFeb 16, 2024 · Fig 4. Bioinformatic analysis pipeline for dual RNA-seq datasets. Quality-filtered RNA-seq reads are aligned in parallel against the respective host and pathogen … WebJan 1, 2024 · Dual RNA-seq reveals a type 6 secretion system-dependent blockage of TNF-α signaling and BicA as a Burkholderia pseudomallei virulence factor important during …

WebApr 29, 2024 · Dual-species transcriptomics or dual-RNA-seq studies use transcriptomics to assess the transcriptional profiles of multiple organisms originating from the same sample . The first dual-species transcriptomics studies were used to analyze interactions between the eukaryotic and prokaryotic organisms in host-pathogen systems [ 16 – 19 ]. WebFeb 8, 2016 · Dual RNA-seq captures both transcriptomes and uses in silico analysis to distinguish species-specific transcripts. Initial sequencing without enrichment or …

WebMar 1, 2024 · Host-Pathogen Transcriptomics by Dual RNA-Seq in Workflows March 1, 2024 8,804 Views Transcriptomics, i.e., the quantification of cellular RNA transcripts, is a … WebApr 29, 2024 · Dual-species transcriptomics or dual-RNA-seq studies use transcriptomics to assess the transcriptional profiles of multiple organisms originating from the same sample [ 15 ]. The first dual-species transcriptomics studies were used to analyze interactions between the eukaryotic and prokaryotic organisms in host-pathogen systems [ 16, 17, 18, …

WebDec 14, 2024 · Here we explored the transcriptome of the pneumococcus and its host using dual species RNA-seq at diverse host anatomical sites in vivo. Blood, kidneys, lungs, and nasopharynx were harvested from mice colonized or infected with three different Spn strains, with uninfected host tissue used as control. These data provide a comprehensive …

WebDual RNA-Seq has been applied to simultaneously profile RNA expression in both the pathogen and host throughout the infection process. This technique enables the study of the dynamic response and interspecies gene regulatory networks in both interaction partners from initial contact through to invasion and the final persistence of the pathogen ... marocchesa azienda agricola marocchi espositoriWebFeb 16, 2024 · “Dual SAGE” refers to the simultaneous analysis of host and pathogen by Serial Analysis of Gene Expression (SAGE), and “Multi RNA-seq” refers to a … marocchi avanzaWebFeb 8, 2016 · To date, dual RNA-seq is one of the most effective tools for understanding host-pathogen interactions [16]. It is useful for investigating global changes in gene … marocchi armi shotgunsWebAug 5, 2024 · Keywords: transcriptomics, dual RNA-seq, host–pathogen interactions, Salmonella enteritidis, duck. Citation: Zhang Y, Song L, Hou L, Cao Z, Vongsangnak W, Zhu … marocche droWebNov 2, 2024 · Dual RNA-Seq is the parallel transcriptomic analysis of bacterial pathogens and their eukaryotic host cells, which generally involves a typical workflow including RNA extraction, rRNA depletion, library preparation, sequencing, and … marocchi armsWebFeb 4, 2024 · Therefore, we used a host–pathogen metabolic modeling approach to analyze our dual RNA-seq data further. We merged our Mtb genome-scale model (sMtb2.0) [ 66 ] with the human alveolar macrophage model [ 30 ] and the Integrative Metabolic Analysis Tool (iMAT) for the integration of transcriptomics data into genome-scale models [ 67 ]. dash miami store