Flow cytometry analysis of rat kidney
WebJun 24, 2024 · Analysis of three different models of kidney injury confirmed that basically all five MNP subsets were contributing to both CD86 + MHCII + CD206- and CD206 + cells. WebOct 25, 2015 · Intracellular flow cytometry. For flow cytometry analysis, differentiated cells were fixed and stained using the Cytofix/Cytoperm Kit (BD) following the manufacturer's instructions. ... Blood Institute (NHLBI) [U01HL100408, U01HL107440 and R21HL120781] and National Institute of Diabetes and Digestive and Kidney Diseases …
Flow cytometry analysis of rat kidney
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WebApr 10, 2024 · Flow cytometry analysis to assess transfection efficiency. To demonstrate transfection efficiency, flow cytometry (Beckman Cytoflex) was performed. ... we assessed the in vivo toxicity of mRNA-cLNP. After tissue repair was completed at 8 weeks, hearts, livers, lungs, and kidneys of three rats in each group (NC, mRNA, mRNA-LNP, mRNA … WebOct 4, 2024 · Cell cycle analysis is crucial to understand cellular responses to changing environmental conditions. Since its inception, flow cytometry has been particularly useful for cell cycle analysis at single cell level due to its speed and precision. Previously, flow cytometric cell cycle analysis relied solely on the measurement of cellular DNA content.
WebAug 1, 2024 · ☛ Using techniques like multi-parametric flow cytometry, flow sorting followed by gene expression, data analysis, RNA sequencing, bone-marrow transplant and chimera studies, in vivo cell ... WebA good correlation was demonstrated between flow cytometry-based and manual classification of rat neutrophils, which suggests that flow cytometry could replace manual classification as part of a ...
WebNov 21, 2013 · Polychromatic flow cytometry is a powerful tool for assessing populations of cells in the kidney through times of homeostasis, disease and tissue … WebJun 24, 2024 · When using the sequence: flow cytometry analysis AND BAL cells AND rat, the total number goes down to 23 available publications, of them, 10 from the 90s (vs. 20 of 91 for mouse literature). Even though …
WebMar 25, 2015 · Using flow cytometry, immunofluorescence, and RT-PCR, we show that our method is suitable for studying and isolating proximal tubular cells with an anti Prominin …
WebIn analyses of images collected from fixed kidney tissue, VTEA replicated the results of flow cytometry while providing detailed analysis of the spatial distribution of immune cells in different regions of the kidney and in relation to specific renal structures. Unbiased exploration with VTEA enabled us to discover a population of tubular ... irs efile system down 2022WebFeb 17, 2024 · Flow cytometry analysis reported low cell mortality during the isolation protocol. There are several factors that may affect cell viability and surface markers … irs efile shutdown 2020 resumeWebJan 14, 2016 · The rat is a commonly used model for immunological investigation. Yet basic research and characterisation of leukocyte populations and sub-sets lags far behind murine research, with inconsistency on reported leukocyte markers and their overlap. These shortcomings limit the opportunity for more complex and advanced rat immunology … irs efile shutdown 2022 resumeWebFeb 10, 2024 · Flow cytometry staining was performed as previously described using specific antibody panels for myeloid cells in rat tissue, which included CD45 (clone OX-1), CD11b/c (clone OX-42), and the granulocyte marker RP-1 [99,100,101] comparable to Ly6g in mouse. These antibodies are equivalent to mouse tissue used in rats for myeloid … irs efile system shutdown 2021WebApr 15, 2024 · Flow cytometry is often used to model cell cycle dynamics of cultured cells, but many commonly used dissociation techniques reduce the yield of intact cells in kidney tissue. Here, we present a new protocol … portable water well drilling rigWebApr 15, 2024 · Flow cytometry studies on injured kidney tubules are complicated by the low yield of nucleated single cells. Furthermore, cell-specific responses such as cell cycle dynamics in vivo have … irs efiling reject error codesWebAug 1, 2016 · Rat peritoneal macrophages were used as positive control for macrophage marker detection by flow cytometry. Our protocol resulted … portable water well pump puller