Cholesterol uptake cell-based assay kit
WebEstablishment of a cell-based assay We have set up a cell-based screening method using fluorescent-labeled LDL uptake in HepG2 cells as an end point assay. We treated human liver HepG2 cells with a ‘gain-of-function’ mutant D374Y-PCSK9 protein at 1 µg/ml concentration (13.4 nM). As shown in WebThis cholesterol efflux assay can be used to: - screen small molecules for their effect on cholesterol efflux as a part of drug discovery program. Cholesterol efflux assay …
Cholesterol uptake cell-based assay kit
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WebMar 5, 2015 · Cholesterol was measured following the protocol provided in the Cholesterol Uptake Cell-Based Assay Kit from Cayman Chemical Company (Ann Arbor, Michigan USA). Briefly, Caco-2 cells were incubated for 40 minutes in glucose free HBSS containing 20μg/ml NBD Cholesterol with or without 10ng/mL LPS, 100nM Aβ 1–40, 1μM Aβ 1–40, ... WebCayman’s Cholesterol Uptake Cell-Based Assay Kit provides a convenient tool for studying cholesterol absorption modulators. The kit employs NBD Cholesterol, a fluorescently …
WebProduct Description. Cholesterol, particularly in the form of low density lipoproteins (LDL), is well understood to be associated with increased risk of coronary heart disease. The … WebCell-based assays are used to analyze several living cell properties. These products may include cell growth and differentiation assays, apoptosis assays, and signal transduction assays. Cell Function Reagents and Kits (387) General Cell Based Assays (324) Cell Structure Reagents and Kits (243) Signal Transduction Reagents and Kits (149)
WebSep 10, 2024 · Then, cholesterol was measured using the Amplex ® Red Cholesterol Assay Kit (Invitrogen) according to the manufacturer’s instructions . The Amplex ® Red assay was developed in a 96-well plate by the reaction of 50 μL of Amplex ® Red working solution (75 μL of a 300 μM of Amplex ® Red reagent and 2 U/mL of horseradish … WebJul 3, 2024 · Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) are associated with obesity. They are accompanied by increased levels of free cholesterol in the liver. Most free cholesterol resides within the plasma membrane. We assessed the impact of adding or removing free cholesterol on the function and …
WebFeb 6, 2024 · Right, competition fluorescent polarization assays to determine the competition of the indicated concentrations of cholesterol, 25-HC, and 7α,25-HC with 22-NBD cholesterol (200 nM) for...
WebCayman’s Cholesterol Cell- Based Detection Assay Kit includes Filipin III, Fixative, and Wash Buffer in a ready to use format. It provides a simple fluorometric method to study … flights from san diego unitedWebCholesterol Uptake Assay Kit - Information Assay Genies total cholesterol assay kit (Fluorometric) is based on the cellular uptake of a fluorescently tagged cholesterol probe. The fluorescence intensity measured at Ex/Em = 485/535 nm is proportional to the amount of cholesterol uptaken by the cells. Applications cherry bomb coffee seatacWebAbout This Assay Cayman’s LDL Uptake Cell-Based Assay Kit provides a convenient tool for studying LDL uptake and regulation at the cellular level. The kit employs human LDL conjugated to DyLight™ 550 (product of Thermo Fisher Scientific Inc.) as a fluorescent probe for detection of LDL uptake into cultured cells. An LDL cherry bomb chords printableWeb2. About the Assay. The EZcount. TM. MTT Cell Assay kit is designed for determination of cell viability and cell proliferation and/or effect of cytotoxic agent. This kit is based on the quantitative measurement of extracellular reduction of the yellow colored water soluble Tetrazolium dye 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium ... cherry bomb coffee coWebProduct overview. Cholesterol/ Cholesteryl Ester Assay Kit ab65359 provides a simple method for sensitive quantification of total cholesterol, free cholesterol, and cholesteryl esters, by colorimetric or fluorometric … flights from sandy to jay vermontWebApr 28, 2014 · For the luciferase assay, 20 μL of cell lysate was transferred to a test tube and assessed directly by means of a Lumat LB 9507 luminometer (Berthold Detection Systems, Pforzheim, Germany), using a luciferase assay kit from Promega. The protein content was quantified using a bicinchoninic acid (BCA) assay (PIERCE, Rockford, IL, … flights from san diego to wichita ksWebBioAssay Systems' cholesterol uptake assay kit is based on cellular uptake of a fluorescently tagged cholesterol probe. The fluorescence intensity measured at λex/em … cherry bomb cocktail recipe